Nitro-Oleic Acid Attenuates Inflammasome Activation and Exerts Cytoprotective Effects in Retinal Pigment Epithelial Cells: Implications for Uveitis Treatment
Purpose
We aimed to elucidate the molecular mechanisms underlying NOOA-mediated protection in retinal pigment epithelial (RPE) cells and explore its potential for uveitis treatment.
Methods
ARPE-19 cells, were treated with either OA, PA or with nitro-OA in the absence or presence of TNF-α , to observe their effects in terms of Cell proliferation assay, Western blot, RT-qPCR and ELISA.
Results
Oleic acid (OA) and palmitic acid (PA) upregulated the expression of inflammasome components, while NOOA ameliorated OA-induced cell toxicity and downregulated tumor necrosis factor-alpha (TNF-α)-induced interleukin-1β (IL-1β) gene and protein expression. Moreover, NOOA treatment attenuated TNF-α-induced nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) hyperphosphorylation and upregulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) antioxidant genes.
Conclusion
Our study reveals that nitro-oleic acid exerts anti-inflammatory and cytoprotective effects on retinal pigment epithelial cells by attenuating inflammasome activation, modulating NFκB signaling, and upregulating antioxidant gene expression. These findings support further investigation of nitro-oleic acid as a potential therapeutic agent for uveitis and other inflammatory eye diseases.
Conflict of interest
No
Authors 1
Last name
CHANG
Initials of first name(s)
YC
Department
Department of Ophthalmology, School of Medicine, College of Medicine, Kaohsiung Medical University
City
Kaohsiung
Country
Taiwan
Authors 2
Last name
Chen
Initials of first name(s)
KJ
Department
Department of Ophthalmology, Kaohsiung Municipal Siaogang Hospital
City
Kaohsiung
Country
Taiwan
Authors 3
Last name
Kao
Initials of first name(s)
TE
Department
Cheng-Ching International Eye Hospital
City
Kaohsiung
Country
Taiwan
Authors 4
Last name
Kao
Initials of first name(s)
YH
Department
Department of Medical Research, E-Da Hospital
City
Kaohsiung
Country
Taiwan
Authors 5
Last name
Lee
Initials of first name(s)
DY
Department
Department of Ophthalmology, Kaohsiung Medical University Hospital
City
Kaohsiung
Country
Taiwan
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