Cytokines profile in ocular fluid of patients with uveitis: preliminary results
Purpose
To understand the pathophysiology of uveitis, we analyzed the intraocular fluid cytokine profile of individuals with uveitis.
Methods
Aqueous/vitreous humor samples of 35 individuals with active uveitis were analyzed: infectious uveitis (IU) [ocular toxoplasmosis (OT) (n=10), herpetic uveitis (HU) ( n=10), other infection ( n=3)] and non-infectious uveitis (NIU)(n=12). Participants undergoing senile cataract surgery were controls (n=2). Twenty cytokines, functionally classified as pro-inflammatory (INFLAMMA, n=12), immunoregulatory (REG, n=5) and repairing (REPAIR, n=3), were analyzed by multiplex microbeads immunoassay. Cytokine functional predominance based on detection rate was determined for each study group.
Results
Cytokines levels and detection rates did not differ among the OT, HU and NIU groups, except for IL-10 in all-group analysis (p=0.019), though not significant in the 2-by-2 analysis (HUvsOT, p=0.057). MCP-1, IP-10 and TGF-β1 were detected in all samples. IL-8 and IL-10 were detected in all uveitic samples. IL-8 levels were 2.9x higher in IU than NIU. VEGF and FGF levels were 2.1x and 1.7x higher in NIU than IU. TNF-α, IL-12p70 and IFN-γ levels were respectively 9.9, 1.5 and 14.9 folds higher in OT than HU. REG/INFLAMMA IL-4/TNF-α (2.4vs0.8, p=0.002) and REPAIR/INFLAMMA FGF-2/TNF-α (9.7vs1.3, p=0.035) level ratios were higher in HUvsOT. On cytokine functional predominance, HU had a predominant REPAIR profile vs INFLAMMA and REG cytokines, while NIU had a predominance of REPAIR vs REG cytokines.
Conclusion
Our preliminary results indicate that OT displays a more diverse and intense INFLAMMA cytokine profile and a potential prominent role for REG cytokines in HU and for REPAIR cytokines in NIU.
Conflict of interest
No
Authors 1
Last name
FERRACIOLI-ODA
Initials of first name(s)
E
Department
Ophthalmology, Faculdade de Medicina, Universidade de Sao Paulo
City
Sao Paulo
Country
Brazil
Authors 2
Last name
Coelho
Initials of first name(s)
V
Department
Laboratory of Immunology, Heart Institute, FMUSP
City
Sao Paulo
Country
Brazil
Authors 3
Last name
Tanaka
Initials of first name(s)
T
Department
Ophthalmology, Faculdade de Medicina, Universidade de Sao Paulo
City
Sao Paulo
Country
Brazil
Authors 4
Last name
Gouveia
Initials of first name(s)
MGS
Department
Clinical and Experimental Gastroenterology Laboratory, LIM 07, FMUSP
City
Sao Paulo
Country
Brazil
Authors 5
Last name
Pinho
Initials of first name(s)
JR
Department
Clinical and Experimental Gastroenterology Laboratory, LIM 07, FMUSP
City
Sao Paulo
Country
Brazil
Authors 6
Last name
Bispo
Initials of first name(s)
PJ
Department
Ophthalmology, Harvard Medical School
City
Boston
Country
United States
Authors 7
Last name
Hirata
Initials of first name(s)
CE
Department
Ophthalmology, Faculdade de Medicina, Universidade de Sao Paulo
City
Sao Paulo
Country
Brazil
Authors 8
Last name
Antonangelo
Initials of first name(s)
L
Department
Laboratorial Medicine Laboratory, LIM 03, FMUSP
City
Sao Paulo
Country
Brazil
Authors 9
Last name
Yamamoto
Initials of first name(s)
JH
Department
Ophthalmology, LIM 33, Faculdade de Medicina, Universidade de Sao Paulo
City
Sao Paulo
Country
Brazil
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