Photobiomodulation (PBM) with Near Infrared Light (670 nm) Provides Anti-Inflammatory and Anti-Apoptotic Effects in a Rat Model of Endotoxin-Induced Uveitis (EIU).
Purpose
The purpose of this study was to investigate the anti-inflammatory and anti-apoptotic effects of near infrared light (670 nm) in a rat model of endotoxin-induced uveitis (EIU). The study aimed to demonstrate the potential of photobiomodulation (PBM) as a non-invasive and cost-effective therapeutic approach for intraocular inflammatory diseases.
Methods
Wistar rats were subjected to EIU induction through intravitreal injection of lipopolysaccharide (LPS). An hour following LPS injection, the rats were exposed to near-infrared light (670 nm) at varying intensities (1500 lux, 3000 lux) for one hour. Retinal function was evaluated using full-field electroretinogram (ffERG), and the rats were sacrificed for further investigation.
Results
The results demonstrated a significant improvement in light-adapted response of the ffERG in a dose-dependent manner after PBM. qPCR revealed that near infrared light (670 nm) significantly reduced pro-inflammatory associated mRNA expression, TNF-α, IL-6, nuclear factor kappa B (Nf-κb), IL-4, IL-13, IL-1β, nf-κb, and Gasdermin D (GSDMD). The NLRP3 inflammasome pathway was also inhibited by near infrared light (670 nm). Furthermore, near infrared light exhibited anti-apoptosis effects by downregulating apoptosis-related genes (p53, Bax) and increasing Bcl2 expression.
Conclusion
In conclusion, PBM with near infrared light (670 nm) demonstrated anti-inflammatory and anti-apoptotic effects in a rat model of EIU. The results suggest that PBM with near infrared light could potentially serve as a non-invasive and cost-effective therapeutic approach for treating intraocular inflammatory diseases.
Conflict of interest
No
Authors 1
Last name
LIU
Initials of first name(s)
P.K.
Department
Department of Ophthalmology, School of Medicine, College of Medicine, Kaohsiung Medical University
City
Kaohsiung City
Country
Taiwan
Authors 2
Last name
Chen
Initials of first name(s)
T.W.
Department
Institute of Eye Research
City
Hualien
Country
Taiwan
Authors 3
Last name
Wen
Initials of first name(s)
Y.T.
Department
Institute of Eye Research
City
Hualien
Country
Taiwan
Authors 4
Last name
Tsai
Initials of first name(s)
R.K.
Department
Institute of Eye Research
City
Hualien
Country
Taiwan
This website uses cookies to ensure you get the best experience on our website.
Learn more