Comprehensive microRNA analysis using vitreous humor of ocular sarcoidosis patients
Purpose
MicroRNAs (miRNAs) are small RNAs and a type of non-coding RNA. More than 2,600 types of miRNAs have already been identified in humans, and their potential as biomarkers for many diseases has been demonstrated. Previously, we performed a comprehensive analysis of serum miRNAs in three major Japanese uveitis cases. In this study, we report a comprehensive analysis of miRNAs in the vitreous of patients with ocular sarcoidosis to search for biomarkers specific to this disease.
Methods
The vitreous humor of 7 patients with ocular sarcoidosis diagnosed at Department of Ophthalmology, Tokyo Medical University Hospital were included in the study. RNA was extracted from the vitreous humor obtained during vitrectomy, and the expression levels of 2,565 miRNAs were comprehensively analyzed using microarrays (DNA chip, 3D-Gene). Vitreous from 10 cases of macular hole and 14ceses of epiretinal membrane and 5 cases of unclassifiable uveitis were used as control to identify miRNAs specific for sarcoidosis.
Results
When compared to macular hole and epiretinal membrane, the expression of 1,353 miRNAs was significantly upregulated and 22 miRNAs were significantly downregulated in sarcoidosis. When compared to uveitis of unknown cause, the expression of 616 miRNAs was significantly upregulated and 18 miRNAs were significantly downregulated.
Conclusion
Comprehensive analysis of miRNAs in vitreous humor identified sarcoidosis-specific miRNAs. Combining the data obtained in this study with serum data serve as a diagnostic marker, since sarcoidosis and uveitis of unknown cause showed different profiles.
Conflict of interest
No
Authors 1
Last name
ASAKAGE
Initials of first name(s)
M
Department
Ophthalmology, Tokyo Medical University Hospital
City
Tokyo
Country
Japan
Authors 2
Last name
Usui
Initials of first name(s)
Y
Department
Ophthalmology, Tokyo Medical University Hospital
City
Tokyo
Country
Japan
Authors 3
Last name
Nezu
Initials of first name(s)
N
Department
Ophthalmology, Tokyo Medical University Hospital
City
Tokyo
Country
Japan
Authors 4
Last name
Shimizu
Initials of first name(s)
H
Department
Ophthalmology, Tokyo Medical University Hospital
City
Tokyo
Country
Japan
Authors 5
Last name
Tsubota
Initials of first name(s)
K
Department
Ophthalmology, Tokyo Medical University Hospital
City
Tokyo
Country
Japan
Authors 6
Last name
Yamakawa
Initials of first name(s)
N
Department
Ophthalmology, Tokyo Medical University Hospital
City
Tokyo
Country
Japan
Authors 7
Last name
Takanashi
Initials of first name(s)
M
Department
Molecular Pathology, Tokyo Medical University
City
Tokyo
Country
Japan
Authors 8
Last name
Kuroda
Initials of first name(s)
M
Department
Molecular Pathology, Tokyo Medical University
City
Tokyo
Country
Japan
Authors 9
Last name
Goto
Initials of first name(s)
H
Department
Ophthalmology, Tokyo Medical University Hospital
City
Tokyo
Country
Japan
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