Peripheral blood transcriptome in ocular sarcoidosis
Purpose
To identify a peripheral blood transcriptome distinguishing participants with ocular sarcoidosis from healthy controls.
Methods
Cross-sectional study of participants enrolled into Biomarkers in Ocular Sarcoidosis Study at the Proctor Foundation/UCSF. At baseline, participants had an ocular examination and peripheral blood samples were collected were collected into PAXgene RNA tubes and stored at -80C. Samples were prepared for metagenomic RNA sequencing with RNA extracted from peripheral blood. Analysis of sequenced data used a rapid computational pipeline to classify host genes. Briefly, quality filtered RNA transcripts were aligned to the ENSEMBL CRCh38 human genome build using STAR. Genes were filtered to include only protein-coding genes that were expressed in at least 25% of the patients. Differentially expressed genes with false discovery rate (FDR) <0.01 were considered as notable.
Results
Ten genes exhibited ≥ 4-fold difference in expression with FDR <0.01 in participants with ocular sarcoidosis compared to controls, including those involved in regulating innate immunity, regulation of T-cells, cell adhesion, apoptosis, or tumor suppression.
Conclusion
This study provides an analysis of the whole blood transcriptional profile of subjects with ocular sarcoidosis, allowing such patients to be distinguished from healthy controls. The peripheral blood transcriptional signature reflects ocular inflammation.
Conflict of interest
No
Authors 1
Last name
GONZALES
Initials of first name(s)
J. A
Department
Francis I. Proctor Foundation
City
San Francisco
Country
United States
Authors 2
Last name
Takhar
Initials of first name(s)
J.
Department
Francis I. Proctor Foundation
City
San Francisco
Country
United States
Authors 3
Last name
Joye
Initials of first name(s)
A.
Department
Francis I. Proctor Foundation
City
San Francisco
Country
United States
Authors 4
Last name
Acharya
Initials of first name(s)
N.R.
Department
Francis I. Proctor Foundation
City
San Francisco
Country
United States
Authors 5
Last name
Doan
Initials of first name(s)
T.A.
Department
Francis I. Proctor Foundation
City
San Francisco
Country
United States
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